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(Solved): Pseudomonas aeruginosa subtype L10 has been isolated from heavily contaminated soil and it can degra ...



Pseudomonas aeruginosa subtype L10 has been isolated from heavily contaminated soil and it can degrade a variety of hydrocarbons. You plan to set-up arlarge scale bioremediation process for soil contaiminated with fossil oil and you need to calculate the cooling requirements. The culture is a suspension culture (oil-contaminated soil particels suspended in water), which, for our purpose, does not make a difference with respect to calculations compared to a microbiological culture in fully soluble substrates. For calculations, hexadecane is used as a proxy for fossil oil. Bacterial growth with ammonia as N-source can be described using the following equation: C16​H34​+aO2​+bNH3​⋯cCH1.99​O0.506​ N0.2​+dH2​O+eCO2​. You want to estimate the cooling requirements per kg fossil oil to estimate the cooling costs. You expect, that the realistic yield is 28% of the theoretical maximal possible biomass yield. The process is performed at 30∘C in an air-flow reactor. The power input required for sparging is neglected. In the process, you loose about 55 kg water per ton substrate consumed due to evaporation. Δhv,30∘C​ is 2430.7 kJ/kg. The biomass contains 4.6% ash.

what is the realistic biomass yield and please do the stoichiometric balance step by step?



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